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- W2364888931 abstract "Pekingmycin is a broad spectrum antibiotic produced by X.nematophila var.pekinense CB6,a bacterial strain carried in intestine of entomopathogenic nematode Steinernema carpocapsae.Previous study showed pekingmycin was suppresive against Phytophthora blight of potato and pepper in fields.In order to improve the Pekingmycin productivity,we treated the parental strain CB6 with ultraviolet(UV),microwave(MW) and nitrosoguanidine(NTG) and cultivated in 96 well microplates for mutants screening.Pekingmycin component was extracted with 35% methanol for 24 h.High activity strains were determined based on UV absorption at 312 wavelength and antibiotic assay against B.subtilis on solid plate.Four mutant strains with high antibiotic activity were screened out and their inhibitory zone was increased by 6.39%-11.94% in comparison with the original strain CB6.Bioassay showed no significant change in inhibitory zone after 6 generations,indicating the mutant strains are genetically stable in highly antibiotic activity." @default.
- W2364888931 created "2016-06-24" @default.
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- W2364888931 date "2013-01-01" @default.
- W2364888931 modified "2023-09-24" @default.
- W2364888931 title "Screening of High Yield Pekingmycin-producing Strains of Xenorhabdus nematophila var. pekinense by Cultivation in 96-well Microtiter Plates" @default.
- W2364888931 hasPublicationYear "2013" @default.
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