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- W2364889585 abstract "In the post-genome era,the combination data with mass spectrometry(MS) provide an effective method for protein identification,and MS has been developed into a powerful tool for proteomics.Now,of Nosema bombycis proteins,only a few have been identified,including some spore wall proteins and polar tube proteins.In order to obtain more information of N.bombycis proteins with big molecular weight,we used the technology of proteomics,LC-MS/MS,in this study to identify and analyze two abundant protein bands of glass bead-extracted total proteins of N.bombycis.We successfully identified a total of 16 proteins in H1(130-170 kDa) band,including polar tube protein 3(PTP3),polar tube protein 2(PTP2),eukaryotic translation initiation factor 2C 2(eIF2C 2),elongation factor 1-alpha(EF1-alpha),elongation factor 2(EF2),actin and some others,thus suggesting that the two structural proteins(PTP2 and PTP3) could form a complex,and the translational related proteins,eukaryotic translation elongation factor 1-alpha(EF1-alpha),elongation factor 2(EF2)and actin could form a big complex too.Treated with SDS or β-Mercatoethanol,these two complexes were stable,which suggested that their ligation is not by the disulfide bond alone.Over 20 proteins were identified in the H2 band with a molecular weight size ranging from 85 kDa to 100 kDa,such as adenosine triphosphatas(ATPase),transitional endoplasmic reticulum ATPase TER94,M1 family aminopeptidase 1,transitional endoplasmic,PTP3,PTP2,eIF2C 2,EF2,EF1-alpha and so on.According to functional analysis,these proteins mainly included the following four aspects: hydrolytic enzymes,protein synthesis and folding,cellular structure and movement.Our research results provide more basic data for proteome and some clues for further research of protein interaction in microsporidia." @default.
- W2364889585 created "2016-06-24" @default.
- W2364889585 creator A5054836635 @default.
- W2364889585 date "2012-01-01" @default.
- W2364889585 modified "2023-09-23" @default.
- W2364889585 title "LC-MS/MS Analysis of Two Main Bands with High Molecular Weight in Total Proteins of Nosema bombycis" @default.
- W2364889585 hasPublicationYear "2012" @default.
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