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- W2364895076 abstract "The prokaryotic expressing vector pAM34 containing Sesbania rostrata phytochelatin synthase1 open reading frame was constructed on the base of pGEX-4T-1 and transferred into E.coli BL21(DE3),then the fused protein GST-SrPCS1 was induced by 0.1mmol/L at 28℃,identified by western blotting and the rabbit was immunized with the purified protein.The results suggested that the fusion protein GST-SrPCS1 was expressed and the titer of the anti-serum was about 1∶2000,indicating that the polyclonal anti-AtPCSl-His serum has high activity and speciality." @default.
- W2364895076 created "2016-06-24" @default.
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- W2364895076 date "2011-01-01" @default.
- W2364895076 modified "2023-09-26" @default.
- W2364895076 title "Prokaryotic Expression of the Recombinant SrPCS1 and Preparation of Polyclonal Anti-serum Against SrPCS1" @default.
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