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- W2364895988 abstract "According to the Lyc-β gene (X86452) and Lyc-e gene (Y14387) sequences published in the GenBank,four pairs of primers were designed to amplify the fragments of 302 and 330 bp (Lyc-β gene),302 and 288 bp (Lyc-e gene) from tomato cDNA. The gusA gene intron sequences of 168 and 235 bp were cloned from the plasmid pCAMBIA-2301 (AF234316). Four plant expression vectors interfering Lyc-β and Lyc-e genes were constructed by ligation the intron fragments of gusA gene within two target gene fragments,which were designed in opposite direction. The transformed tobacco plants were identified by PCR,and a total of 107 transgenic plants were obtained. The interference effects were analyzed by real time PCR,and the results showed that the target genic relative abundance in the transgenic plants were 3. 4% ,16. 4% ,15. 2% and 21. 8% respectively compared with wild type plants. Further analysis of the pigment content in transgene plants showed that the average accumulating rate of lycopene content in transgenic plants were 3. 4, 2. 1,3. 4,2. 0μg /g,respectively. The β-carotene and lutein contents in transgenic plants had the corresponding change." @default.
- W2364895988 created "2016-06-24" @default.
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- W2364895988 date "2010-01-01" @default.
- W2364895988 modified "2023-09-25" @default.
- W2364895988 title "CONSTRUCTION OF RNAI PLANT EXPRESSION VECTORS FOR INTERFERING LYCOPENE CYCLASE-β/ε GENES AND ITS EXPRESSION ANALYSIS" @default.
- W2364895988 hasPublicationYear "2010" @default.
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