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- W2364897536 abstract "Objective To study the mechanism of dermal fibroblasts as a feeder layer to support the growth of human keratinocytes. Methods Human dermis fibroblasts were isolated and cultured and then treated with mitomycin-C. The expression of type Ⅰand type Ⅲ precollagen mRNA and relevant protein in feeder layer were examined by RT-PCR and Immunohistochemistry. KCs were cultured both on FB and NIH3T3 feed layer as control, the adhering numbers and the time of fusion were recorded. Results RT-PCR showed an increase of type Ⅰprecollagen mRNA in FB feeder layer as compared with that of normal fibroblasts (P0.05)and no change was found in type Ⅲ precollagen. Positive stains of collagen type Ⅰ and Ⅲ were shown in the FB feeder layer and those KCs grew and differentiated well on the FB feeder layer seeded at appropriate densities. There was no statistical difference in the adhering cell numbers and the time of fusion in two different culture feeder layer. Conclusion Human dermal fibroblasts as the feeder layer could secrete collagen type Ⅰ and Ⅲ which will support the multi-layered sheets of cultured keratinocytes." @default.
- W2364897536 created "2016-06-24" @default.
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- W2364897536 date "2007-01-01" @default.
- W2364897536 modified "2023-09-27" @default.
- W2364897536 title "Human dermal fibroblasts as a feeder layer promote the growth of human keratinocytes" @default.
- W2364897536 hasPublicationYear "2007" @default.
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