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- W2364897635 abstract "Conjugated linoleic acids(CLA) are the positional and geometric isomers of linoleic acid.Because of their biofunctions,the researches of CLA have drawn much attention.The simple and efficient analytical method of CLA is the gas chromatography.The analytical method can determine their composition and relative concentration.The first step of gas chromatography analysis is the methyl esterification of the fatty acids.The methyl esterification methods can be classified into three types: acid-catalyzed method,base-catalyzed method and trimethylsilyldiazomethane(TMS) method.Generally,free fatty acids can be methylated by acid-catalyzed method or TMS method;triglycerides can be methylated by acid-catalyzed method or base-catalyzed method.The differences of these three methods in analysis of CLA were studied by thin lay chromatography(TLC) and gas chromatography(GC).The results showed that different methyl esterification methods gave different levels of methyl esterification of fatty acids and the composition of isomers of CLA also changed after methyl esterification.The concentration of fatty acids in the same CLA by acid-catalyzed method was 73.34%,and TMS method was 82.47%.The concentration of tt-CLA by acid-catalyzed method was 24.66%." @default.
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- W2364897635 date "2007-01-01" @default.
- W2364897635 modified "2023-09-27" @default.
- W2364897635 title "Effect of fatty acid methyl esterification methods on analysis of conjugated linoleic acids" @default.
- W2364897635 hasPublicationYear "2007" @default.
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