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- W2364902703 abstract "This study tried to initially establish a method for counting Bifidobacterium longum in yogurt by using polymerase chain reaction(PCR) technique and traditional plate count method technique.Based on the conserved sequence of 16S rDNA sequences of bacteria,a pair of universal primer were designed as a positive mark.And a pair of special primers were also designed and synthesized according to 16S～23S rRNA gene of the Bifidobacterium longum.The randomly selected colonies of the mixed fermented yogurt culturing on BBL agar plate were abstracted and identified by PCR.PCR technology combines with the traditional plate count method technology,established the method for counting the living Bifidobacterium longum in the mixed fermented yogurt.Operation of extraction method must be simple,efficient,responsive and accurate,and it determined the efficient of PCR counting method.This study focused on the factors that influencing the bacterial DNA extraction,as well as the versatility of the bacterial DNA extraction,but also the specificity of the PCR primers for Bifidobacterium longum.A method for counting Bifidobacterium longum in yogurt was initially established by using polymerase chain reaction(PCR) technique and traditional plate count method technique." @default.
- W2364902703 created "2016-06-24" @default.
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- W2364902703 date "2012-01-01" @default.
- W2364902703 modified "2023-09-25" @default.
- W2364902703 title "Study on a new method for counting Bifidobacterium longum in yogurt by PCR" @default.
- W2364902703 hasPublicationYear "2012" @default.
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