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- W2364902959 abstract "Objective To investigate the TCR Vβ gene repertoire, and to clonal expansion of T cells and T cell strains from peripheral blood of patients with T-cell acute lymphoblastic leukemia (T-ALL). Methods The complementarity determining region 3 (CDR3) of TCR Vβ 24 subfamily genes in peripheral blood mononuclear cell from 6 cases with untreated T-ALL and 6 T cell strains were amplified using RT-PCR. The PCR products were further labelled by fluorescein and then analyzed by genescan technique for detecting the CDR3 length, and as a result, the clonality of T cells was determined. Some monoclonal PCR products from T cell strains were analyzed by sequencing to define the sequence of CDR3. Results Six T-ALL cases expressed 5-12 Vβ subfamilies while normal individuals expressed all 24 Vβ subfamilies in peripheral blood. Oligoclonal and biclonal expansion of T cells in one or more Vβ subfamilies were found in each of the 6 cases. In addition, polyclonal patterns in some Vβ subfamilies appeared the trend of oligoclonal expansion. Only one Vβ subfamily's monoclonal expanded T cell could be identified in most T cell strains. Sequences from different T cell strains displayed different CDR3 length and sequences. Conclusion Restrictive changes of TCR Vβ repertoire could be found in peripheral blood T cells from patients with T-ALL.The clonal expansions of T cells are detected in the 6 cases with T-ALL. However, their property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. The results indicate that the identification of TCR Vβ repertoire and clonality of T cells in T-ALL may play a certain role in the detection of minimal residual disease and the design of idiotypic anti-leukemia vaccine." @default.
- W2364902959 created "2016-06-24" @default.
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- W2364902959 date "2004-01-01" @default.
- W2364902959 modified "2023-09-25" @default.
- W2364902959 title "Clonality and gene repertoire analysis of T-cell receptor Vβ associated with T-ALL and T cell strains" @default.
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