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- W2364907566 abstract "Objective To establish an HCV subgenomic replicons cell model that is labeled with reporter gene and to provide a useful tool for HCV research.Methods An HCV subgenomic replicons cell model [HCV-firefly luciferase(Fluc)-Ubi-Gaussia luciferase(Gluc) ]containing dual luciferase reporters was established by monoclonal selection and lentivirus infections.The reporter and HCV protein expression were confirmed by luciferase activity assay and Western blotting,respectively.The efficacy of the cell model was validated by IFN-α which was shown to inhibit HCV replication.Results Luciferase activity and HCV NS5A protein were detected by luciferase activity assay and Western blotting,respectively.Subcellular localization of yellow fluorescence protein(YFP)-MAVS was transferred from mitochondria to nucleis by cleavage of NS3 /4A protease in HCV replicons cells.HCV proteins and luciferase activity were diminished obviously after IFN-α treatment.Conclusion In this cell model,the HCV RNA and host cells are labeled with firefly luciferase and Gaussia luciferase,respectively.The mold can indicate the level of HCV replication and eliminate the cytotoxicity antivirus drugs induced effectively.It will be a valuable tool for studying HCV pathogenesis and screening antiviral drugs." @default.
- W2364907566 created "2016-06-24" @default.
- W2364907566 creator A5019803731 @default.
- W2364907566 date "2013-01-01" @default.
- W2364907566 modified "2023-09-28" @default.
- W2364907566 title "Establishment of a hepatitis C virus subgenomic replicons cell model labeled with dual luciferase reporter" @default.
- W2364907566 hasPublicationYear "2013" @default.
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