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- W2364921215 abstract "OBJECTIVE:To clone the transcriptional regulatory regions of invasion and metastasis associated Ezrin gene in human esophageal carcinoma cells,identify promoter activities of the cloned regions and perform the primary bioinformatics analysis.METHODS:The GC content and CpG islands analysis and potential transcription factor binding sites prediction of Ezrin gene possible transcriptional regulatory region were performed by Web sites.-1 759/+134 and-726/+134 regions of Ezrin gene were cloned from esophageal carcinoma cells genomic DNA to construct recombinant plasmids pGLB-hE(-1 759/+134)and pGLB-hE(-726/+134)by PCR method,and the two regions' promoter activities were detected by the reporter luciferase activity assay.RESULTS:There was a highly GC-rich content with CpG islands in Ezrin gene 5'-flanking region.The region lacked a typical TATA box but Sp1 binding sites were ubiquitous.pGLB-hE(-726/+134)displayed a strong luciferase activity compared with pGLB,while the reporter activity of pGLB-hE(-1 759/+134)was twifold of that of pGLB-hE(-726/+134).CONCLUSION:The-726/+134 region has promoter activity containing core promoter and regulatory promoter regions of Ezrin gene,and the-1 759/-726 region enhances promoter activity containing an enhancer element region of Ezrin gene." @default.
- W2364921215 created "2016-06-24" @default.
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- W2364921215 date "2008-01-01" @default.
- W2364921215 modified "2023-09-28" @default.
- W2364921215 title "Cloning of Ezrin gene transcriptional regulatory region and identification of its promoter activity in esophageal carcinoma cells" @default.
- W2364921215 hasPublicationYear "2008" @default.
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