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- W2364941454 abstract "Objective To explore the proliferative role of leptin and the mechanism underlying this action in huamn breast cancer cell line MCF-7.Methods The expression of leptin receptor(OB-Rb) in MCF-7 cells were detected by fluoroimmunoassay.The cells after serum starvation,were treated by leptin with various concentrations(0,10,50,100,150ng/ml)for different times(6,12,24 h).The effect of lep- tin treatment on cell proliferation was examined by MTT assay.Meanwhile,inhibitory effect of JAK2/ STAT3 inhibitor AG490 or ERK1/2 inhibitor PD98059 on the proliferation of MCF-7 induced by leptin was also studied.MCF-7 were treated with 100ng/ml leptin for various periods(0,20,40,60 rain),the levels of STAT3 phosphorylation and ERK1/2 phosphorylation were examined by Western blot.Results Fluoro- immunoassay showed the presence of leptin receptor OB-Rb in MCF-7.Leptin stimulated the proliferation of MCF-7 in a time-and dose-dependent manner,and the effect was maximum at 100ng/ml for 24h treat- ment,there was no diference between 100 μg/L and 150 μg/L(P0.05).AG490 or PD98059 was sig- nificantly reduced the proliferation stimulation of MCF-7 by leptin in a dose-dependent manner.STAT3 phosphorylation and ERK1/2 phosphorylation were significantly increase in MCF-7 stimulated by leptin and lasted for 60min at least.Conclusion It is suggested that the promotion of huamn breast cancer cell line MCF-7 proliferation by leptin involves activation of JAK2/STAT3 and ERK1/2 signaling pathways." @default.
- W2364941454 created "2016-06-24" @default.
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- W2364941454 date "2007-01-01" @default.
- W2364941454 modified "2023-09-23" @default.
- W2364941454 title "Leptin activates STAT3 and ERK 1/2 pathways and induces human breast cancer cell line MCF-7 proliferation" @default.
- W2364941454 hasPublicationYear "2007" @default.
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