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- W2364942942 abstract "Bioinformatics software was used to analyze the gene of glycoprotein extracellular domain from rabies virus aG strain,and optimize E.coli rare codon of this sequence into E.coli normal codon by codon optimization strategies in this study.The optimized sequence was synthesized and constructed into prokaryotic expression vector pET-28a(+) and transformed into the expression system of Rosetta(DE3) inducted by IPTG.The recombinant protein of glycoprotein extracellular domain was analyzed by SDS-PAGE and Western blotting.Meanwhile,the best expression conditions were studied,and the results indicated that recombinant proteins reached a maximum level when the recombinant bacteria at the condition of 30℃,final concentration of IPTG at 0.6 mM and the time of induction by IPTG at 6 h.This study shows that extracellular domain aG strain of the rabies virus glycoprotein can be successfully expressed in E.coli by codon optimization and expression products have good reactogenicity.The study paves the way for the development of ELISA kits to detect the rabies virus or antigens of rabies virus." @default.
- W2364942942 created "2016-06-24" @default.
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- W2364942942 date "2012-01-01" @default.
- W2364942942 modified "2023-09-24" @default.
- W2364942942 title "Codon optimization,prokaryotic expression and purification of glycoprotein extracellular domain of rabies virus" @default.
- W2364942942 hasPublicationYear "2012" @default.
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