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- W2364961031 abstract "The quick augment of serum albumin gene in human urine may induce renal diseases.The study on human serum albumin by dynamic light scattering technique is helpful to the discovery of inchoate renal diseases.In this paper,the physical model of human serum albumin gene is analyzed firstly.And then the dynamic light scattering experiment setup based on single-mode fibers is constructed,and the water solutions of human serum albumin required in experiments are prepared.The diffusion coefficient of human serum album was measured at different concentrations and pH by means of the above-mentioned setup.Experimental and analytic results indicate that coulomb interactions between the charged proteins acted as main contribution to the diffusion coefficient;the effect of coulomb vanished at the isoelectric(5.2),and the diffusion coefficient of protein is close to minimal.At the isoelectric,the diffusion coefficient decreased linearly with concentration increase.In the concentration range of 5 mg/mL～40 mg/mL,when 532 nm light was used,it was found that Dm=D0[1-(0.00194±0.00008)],where D0=(6.74±0.01)×10-7 cm2/s extrapolated to zero concentration at 23 ℃ and C is in units of mg/mL.The measured diameter of human serum albumin is(3.44±0.01) nm." @default.
- W2364961031 created "2016-06-24" @default.
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- W2364961031 date "2008-01-01" @default.
- W2364961031 modified "2023-09-28" @default.
- W2364961031 title "A Study on Human Serum Albumin Gene Based On Fiber Dynamic Light Scattering" @default.
- W2364961031 hasPublicationYear "2008" @default.
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