FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2364961904> ?p ?o ?g. }

• W2364961904 abstract "Siraitia grosvenorii( Luohanguo) is a herbaceous perennial medicinal and sweetener plant native in Guangxi of China. It has long been used in traditional Chinese medicine as a natural sweetener and also as a folk medicine for the treatment of lung congestion,colds and sore throats. Many cucurbitane-type triterpene glycosides have been isolated and characterized from S. grosvenorii. The active components responsible for the sweetness are the mogrosides,which are members of the family of triterpene glycosides. Mogroside V has an important prospect as natural and low calorie sweetener,which is nearly 425 times sweeter than sucrose. S. grosvenorii currently depends totally on cultivation in China. It's limitedly applied because the narrow distribution,serious continuous cropping obstacle,low content and high extraction costs of mogroside Ⅴ. In order to reduce blindness research and determine the appropriate sequencing strategy,the genome survey before large-scale genome sequencing is needed. This survey can provide information about the size and complexity of the whole genome of the S. grosvenorii. The next generation sequencing technology which has been emerged as a cost effective approach for high-through-put sequence determination has dramatically improved the efficiency and speed of genes discovery and genome research. Genome sequencing of S. grosvenorii has the vital significance to reveal the molecular mechanism of yield,content,growth,pest and disease resistance,and provides an efficient approach to improve content and reduce cost of mogroside Ⅴ by molecular breeding. In this study,the genome size of S. grosvenorii was determined by next-generation sequencing technologies( NGS,Illumina HiseqTM2000). The hybridity percentage,repeats,and GC depth were also estimated by bioinformatics analysis. The results were as follows:( 1) Two DNA libraries of 170 bp and 500 bp are constructed. After cleaning and quality checks,more than 18. 1 Gb high quality data from the genome is generated,which were assembled into 943 296 contigs and 433 325 scaffolds by SOAP denovo software. The contig and scaffold numbers of the length more than 2 kb were 17 855 and 27 993 separately. The longest length of contig and scaffold were 29 kb and 268 kb. The N50 length of contig and scaffold were 484 bp and 2 331 bp.The average genome size and sequencing coverage depth of S. grosvenorii was about 344. 95 Mb and 52 times respectively;( 2) The genome of S. grosvernrii had obvious hybridity peak by K-mer method,the hybridity percentage as high as1. 5%. The assembly results showed that the length of contig N50 and scaffold N50 are much shorter than expected.High hybridity percentage of the genome leads to apparently unusual phenomenon between average depth and GC content,and had a low depth distribution area. There was a weak repeat peak behind the main peak,which demonstrated that S. grosvenorii has more repetitive sequences;( 3) Whole-genome shotgun sequencing( WGS) should not be used to S. grosvenorii genome sequencing separately,and the Fosmid-to-Fosmid or BAC-to-BAC library could be combinational used for better results. This study would not only obtain the basic resources of genome,but also provide a theoretical basis and target genes for S. grosvenorii in transgenic breeding and genetic engineering." @default.
• W2364961904 created "2016-06-24" @default.
• W2364961904 creator A5036682412 @default.
• W2364961904 date "2015-01-01" @default.
• W2364961904 modified "2023-09-26" @default.
• W2364961904 title "Genome survey analysis in Siraitia grosvenorii" @default.
• W2364961904 hasPublicationYear "2015" @default.
• W2364961904 type Work @default.
• W2364961904 sameAs 2364961904 @default.
• W2364961904 citedByCount "0" @default.
• W2364961904 crossrefType "journal-article" @default.
• W2364961904 hasAuthorship W2364961904A5036682412 @default.
• W2364961904 hasConcept C104317684 @default.
• W2364961904 hasConcept C141231307 @default.
• W2364961904 hasConcept C142724271 @default.
• W2364961904 hasConcept C150903083 @default.
• W2364961904 hasConcept C204787440 @default.
• W2364961904 hasConcept C2780527577 @default.
• W2364961904 hasConcept C54355233 @default.
• W2364961904 hasConcept C71924100 @default.
• W2364961904 hasConcept C86803240 @default.
• W2364961904 hasConceptScore W2364961904C104317684 @default.
• W2364961904 hasConceptScore W2364961904C141231307 @default.
• W2364961904 hasConceptScore W2364961904C142724271 @default.
• W2364961904 hasConceptScore W2364961904C150903083 @default.
• W2364961904 hasConceptScore W2364961904C204787440 @default.
• W2364961904 hasConceptScore W2364961904C2780527577 @default.
• W2364961904 hasConceptScore W2364961904C54355233 @default.
• W2364961904 hasConceptScore W2364961904C71924100 @default.
• W2364961904 hasConceptScore W2364961904C86803240 @default.
• W2364961904 hasLocation W23649619041 @default.
• W2364961904 hasOpenAccess W2364961904 @default.
• W2364961904 hasPrimaryLocation W23649619041 @default.
• W2364961904 hasRelatedWork W106428188 @default.
• W2364961904 hasRelatedWork W114769973 @default.
• W2364961904 hasRelatedWork W2002883632 @default.
• W2364961904 hasRelatedWork W2004143253 @default.
• W2364961904 hasRelatedWork W2011111965 @default.
• W2364961904 hasRelatedWork W2108742089 @default.
• W2364961904 hasRelatedWork W2154701173 @default.
• W2364961904 hasRelatedWork W2410414059 @default.
• W2364961904 hasRelatedWork W2463203885 @default.
• W2364961904 hasRelatedWork W2624533138 @default.
• W2364961904 hasRelatedWork W2778917343 @default.
• W2364961904 hasRelatedWork W2897728111 @default.
• W2364961904 hasRelatedWork W2902115059 @default.
• W2364961904 hasRelatedWork W2916114485 @default.
• W2364961904 hasRelatedWork W2951181326 @default.
• W2364961904 hasRelatedWork W2955548509 @default.
• W2364961904 hasRelatedWork W2969481421 @default.
• W2364961904 hasRelatedWork W3088056938 @default.
• W2364961904 hasRelatedWork W3124129973 @default.
• W2364961904 hasRelatedWork W3105328993 @default.
• W2364961904 isParatext "false" @default.
• W2364961904 isRetracted "false" @default.
• W2364961904 magId "2364961904" @default.
• W2364961904 workType "article" @default.