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- W2364966029 abstract "Objective To study the differences of different methods to culture rat bone marrow mesenchymal stem cells(BMSCs).To get morphological observation and identification of the BMSCs that was cultured and proliferated.Methods Harvested bone marrow from 2 SD rats' femur and tibia.Whole bone marrow culture and density gradient centrifugation culture were used respectively.To observe the morphology in different generations of the cells cultured in two different methods.The number of cells in each generation was determined.Cell surface marker such as CD34,CD29,CD44,and CD45 in P5 generation cells were detected by flow cytometry.Results Cells acquired from two culture methods were long fusiform,showed characteristic swirling growth.Cell's proliferation was faster in whole bone marrow culture.Expression rates of cell surface markers CD29,CD34,CD44,and CD45 from the cells obtained from whole bone marrow culture were 91% ,4.3% ,87.1% ,and 0.1% .Expression rates of cell surface markers CD29,CD34,CD44,and CD45 from density gradient centrifugation culture were 96.9% ,3.6% ,89.7% ,and 0% .Conclusion The study proves the cells cultured by both methods are in line with characteristics of BMSCs.The whole bone marrow culture compared with the density gradient centrifugation culture has not only successfully isolated BMSCs but also more economical and simple to operate and easier to get a lot of BMSCs." @default.
- W2364966029 created "2016-06-24" @default.
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- W2364966029 date "2013-01-01" @default.
- W2364966029 modified "2023-09-26" @default.
- W2364966029 title "Comparison of different culture methods for rat bone marrow mesenchymal stem cells and cultured cell identification" @default.
- W2364966029 hasPublicationYear "2013" @default.
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