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- W2364989935 abstract "Objective:To investigate the effect of hypoxia on the differentiation of rat genioglossus myoblasts.Method:According to the oxygen concentration of culture environment,genioglossus myoblasts were divided into two groups:the normal oxygen concentration group(NC)(21 %) and hypoxia group(HG)(1 %) and medium was changed to 2 % horse serum to induce differentiation.The mRNA levels of MyoD,myogenin and MHC were determined by real-time RT-PCR and the protein contents of which were detected by western blotting analysis;Cellmorphology was observed by inverted microscope.Result:During differentiation the protein levels of HIF-1α were significantly increased,however the mRNA levels stayed unchanged.Hypoxia significantly decreased the mRNA and protein levels of MyoD,myogenin and MHC,which was coincidence with the results of cellular morphology observation.Conclusion:Hypoxia may inhibited the differentiation of rat genioglossus myoblasts through upregulating HIF-1αexpression,which postponed or inhibitedgenioglossus lesion repair." @default.
- W2364989935 created "2016-06-24" @default.
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- W2364989935 date "2012-01-01" @default.
- W2364989935 modified "2023-09-22" @default.
- W2364989935 title "Effect of hypoxia on the differentiation of rat genioglossus myoblast" @default.
- W2364989935 hasPublicationYear "2012" @default.
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