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- W2365093876 abstract "Objective To develop,optimize and characterize tissue engineered oral mucosa equivalents made of an epithelium layer on 4 different scaffolds. Methods Fibroblasts and keratinocytes were isolated and cultured from mucosa biopsies obtained from healthy volunteers.After amplification,fibroblasts were seeded into de-epidermized human dermis(DED)and Alloderm by centrifugation.The collagen based scaffolds were prepared by seeding fibroblasts directly.After one week culture,the keratinocytes were seeded on top of the dermis and cultured for another 11 days.The equivalents were all prepared for histology. Results General histology showed that multilayered,stratified and differentiated epithelia were formed on all 4 different scaffolds.Oral epithelia cultured on DED closely attached to the dermis,and formed obvious rete pegs resembling normal oral epithelium.Different from previous reports,the epithelia cultured on the Alloderm did not attach tightly to the substrate.The epithelia generated on the fibroblast-populated collagen scaffold showed a fully stratified structure with apparent cornification.The epithelia formed on the collagen sponge-collagen gel complex invaded into the small gaps that were not filled with collagen gel. Conclusion Oral keratinocytes cultured on DED and collagen gel exhibited tissue organization that resembled normal oral mucosa.For the latter one limited clinical use is possible due to dramatic shrinkage and reduced stability." @default.
- W2365093876 created "2016-06-24" @default.
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- W2365093876 date "2011-01-01" @default.
- W2365093876 modified "2023-09-26" @default.
- W2365093876 title "Characteristics of Tissue Engineered Oral Mucosa Based on Four Different Scaffolds" @default.
- W2365093876 hasPublicationYear "2011" @default.
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