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- W2365105836 abstract "A recombinant adenoviral vector harbouring and coexpressing green fluorescent protein (GFP) and glial cell linederived neurotrophic factor (GDNF) was constructed. The construct AdGDNFEGFP was microinjected into the cortex cerebri,corpus striatum,lumbus segment of medulla spinalis,cisternae subarachnoideales and substantia grisea of medulla spinalis of rats. Then the distribution and expression of the exogenous genes in different types of neural cells were observed under fluorescent microscope by using GFP as an indicator. The recombinant virus was used to transfect rat/mouse cell lines NSC and NIH3T3 cells in vitro. The bioengineered cell line coexpressing GDNF and GFP was preliminarily abtained. According to the experimented results,the biosafety and the prospective of applying the adenovector thus constructed to clinical treatment of nervous system diseases were discussed." @default.
- W2365105836 created "2016-06-24" @default.
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- W2365105836 date "2003-01-01" @default.
- W2365105836 modified "2023-09-24" @default.
- W2365105836 title "Construction of a GFP-labeled Recombinant Adenovirus Harbouring GDNF and Its Expression in Rat Neural Cells in vitro and in vivo" @default.
- W2365105836 hasPublicationYear "2003" @default.
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