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- W2365119895 abstract "A simple and useful method for studying engineered strain metabolizing pyruvic acid is critical to metabolism flux research in the metabolism engineering and modern gene engineering.Based on the carbonylation between pyruvic acid and 2,4- Dinitrophenylhydrazine,after clarifying the influcence of pH,temperature and reagent ratio, a method considering the optimal constitutes and reaction conditions was constructed.The common 18 materials found in culture medium,TCA cycle or fermentation products hardly interfere the carbonylation between pyruvic acid and 2,4-Dinitrophenylhydrazine.The ability to consume pyruvid acid of engineered E.coli BL21(DE3)pLysS containing pET22b-pflAB was successfule studied further proved the new method." @default.
- W2365119895 created "2016-06-24" @default.
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- W2365119895 date "2008-01-01" @default.
- W2365119895 modified "2023-09-25" @default.
- W2365119895 title "One method for studying engineered strain metabolizing pyruvic acid" @default.
- W2365119895 hasPublicationYear "2008" @default.
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