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- W2365158981 abstract "In order to determine malachite green(MG) residues in food,the synthesis of antigen and preparation of polyclonal antibody for MG were explored.In this study,carboxylic malachite green(CMG) hapten was first synthesized and analyzed by LC-MS.The hapten was coupled separately with the two carrier proteins BSA and OVA by diazotization method,and identified by UV-visible spectroscopy method.Compared with CMG standard,BSA and OVA,the absorption peaks CMG-BSA and CMG-OVA displayed a marked shift,indicating that the successful conjugation between CMG and BSA or OVA,and the coupling ratio of the immunogen CMG-BSA was calculated to be 10.6:1.Polyclonal antibody for MG was prepared by immunization with CMG-BSA and evaluated using the titer of anti-CMG antisera determined by indirect ELISA.Furthermore,two rabbits were used to evaluate antibody specificity with determined IC50 values of 8.1 ng/mL and 4.6 ng/mL,respectively." @default.
- W2365158981 created "2016-06-24" @default.
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- W2365158981 date "2011-01-01" @default.
- W2365158981 modified "2023-09-23" @default.
- W2365158981 title "Synthesis of Artificial Antigen and Preparation of Polyclonal Antibody for Malachite Green" @default.
- W2365158981 hasPublicationYear "2011" @default.
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