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- W2365171837 abstract "Objective In order to compare various detection methods for human immunodeficiency virus type 2(HIV-2), 16 cases of suspected HIV-2 infections were follow-up investigated.Methods Blood samples were collected from 16 people whose sera were previously found to be HIV-2 antibody positive by using of a HIV-1/2 Western Blot (WB) assay, then analyzed for HIV-2 antibody and nucleic acid.HIV antibody in plasma was screened by HIV-1/2 ELISA.The positive samples were analyzed by a HIV-1/2 Line Immuno Assay(LIA)and a HIV-2 WB, respectively.Proviral DNA in PBMC was detected by HIV-2 specific nested PCR.Results All of the 16 plasma samples were HIV antibody positive by HIV-1/2 ELISA.Using the HIV-1/2 LIA, all samples were HIV-1 antibody positive; 15 were HIV-2 antibody negative and one indeterminate.Using the HIV-2 WB, 3 samples were HIV-2 antibody positive and 13 indeterminate.Since these samples were collected after more than one year following the first tests, peoples with the above indeterminate results were considered to be HIV-2 antibody negative.In addition, all the PBMC samples were negative for HIV-2 by nested PCR.Conclusions All of the 16 suspected HIV-2 infections are infected only with HIV-1.The results of the HIV-1/2 LIA were well concordant with those of HIV-2 specific nested PCR, but the HIV-2 WB produces a number of indeterminate results and some false-positive ones." @default.
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- W2365171837 date "2006-01-01" @default.
- W2365171837 modified "2023-09-25" @default.
- W2365171837 title "A comparing study of various detection methods for human immunodeficiency virus type 2" @default.
- W2365171837 hasPublicationYear "2006" @default.
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