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- W2365175034 abstract "The 5′ and 3′ terminal sequences were determined in order to analyze the relationship between secondary structures and virulence of two dengue type 2 virus strains whose neurovirulence in suckling mice was different.Total RNA was isolated from C6/36 cells infected by D2|04 and brains of suckling mice infected by D2|44.With this RNA as template,the cDNAs of both 5′ and 3′ termini of D2|04 and D2|44 were amplified using RACE methods,respectively.The cDNAs were inserted into pGEM|T vector and then the nucleotide sequences of the cDNA fragments inserted were determined.The secondary structures of 5′ and 3′ termini of D2|04 were predicted with RNAdraw.The 5′ and 3′ untranslated regions of D2|04 and D2 44 were 96 and 454 bases in length,respectively.A C to T change of 5′UTR that distinguished D2|04 from D2|44 at position 59 was predicted to change secondary structures.15 nucleotides of 3′UTR were different between D2|04 and D2|44.T to A(355) and T to G(326) that lay in CS1 and CS2A of 3′UTR were predicted to change secondary structures.The three sites may be relevant to virulence of dengue virus." @default.
- W2365175034 created "2016-06-24" @default.
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- W2365175034 date "2001-01-01" @default.
- W2365175034 modified "2023-09-25" @default.
- W2365175034 title "Sequence Determination and Analysis of the 5′ and 3′ Terminal Regions of Two Dengue Type 2 Virus Strains Isolated in China" @default.
- W2365175034 hasPublicationYear "2001" @default.
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