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• W2365195189 abstract "To test immunoprotection elicited by eucaryotic expression vector of co-encoding SjFerritin gene(SjFer)and granulocyte macrophage colony-stimulating factor(GM-CSF)against challenge infection by Schistosoma japonicum in mice,the special primers were designed according to the sequence of SjFer in GenBank,and SjFer was amplified from S.japonicum adult worm cDNA library.Eighty-five female mice were randomly divided into 5 groups,immunized with 50 μL normal salt,50 μg pcDNA3.0,50 μg pcDNA3.0/GM-CSF,50 μg pcDNA3.0/SjFer and 50 μg pcDNA3.0/SjFer-GM-CSF per mouse in 0,2nd and 4th week,respectively.Sera were collected before immunization and challenge infection,respectively.Levels of specific antibody were detected by ELISA.Lymph-spleencytes of mice were collected two weeks after final immunization.Activity of Lymph-spleencyte proliferation was detected by MTT assay.Expression of recombinant plasmids in murine muscular tissue was detected with immunohistochemistry.Mice were challenged with 40±1 S.japonicum cercaria per mouse 2 weeks after the final vaccination.Forty-five days later,mice were killed and perfused,and the adult worms and eggs were counted.Results showed that pcDNA3.0/SjFer,pcDNA3.0/GM-CSF and pcDNA3.0/SjFer-GM-CSF expressed in murine muscular tissue.The serium IgG level did not change obviously in control groups(P 0.05),but increased significantly in experimemtal groups(P 0.01).Furthermore,the serium IgG level in group immunizated with pcDNA3.0/SjFer-GM-CSF was higher than that with pcDNA3.0/SjFer(P 0.01).The proliferation activity of spleen T lymphocytes detected by MTT assay did not change obviously in control groups(P 0.05)and increased significantly in experimental groups after inducation of ConA or rSjFer(P 0.01).The proliferation activity of spleen T lymphocytes in the group immunizated with pcDNA3.0/SjFer-GM-CSF were higher than that with pcDNA3.0/SjFer(P 0.01).The worm reduction rate and egg reduction rate was 36.27 %,39.22 % and 36.10 %,49.04 % in groups immunization with pcDNA3.0/SjFer and pcDNA3.0/SjFer-GM-CSF,respectively.In conclusion,GM-CSF can improve humoral,cellular immunity and immunoprotection elicited by eucaryotic expression vector encoding SjFer in mice." @default.
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• W2365195189 date "2007-01-01" @default.
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• W2365195189 title "Immunoprotection Elicited by Eucaryotic Expression Vector of Co-encoding SjFer and GM-CSF in Mice" @default.
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