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- W2365202871 abstract "Aim To obtain the recombinant antigen used for detection of dengue virus infection Mothed The Dengue virus NGC strain,serotype 2 virus,was cultured through infected the C6/36 monolayer cell The viral RNA was extracted from the cultured supernatant The artificial synthesized primers were applied to amplify the viral envelope antigenic gene fragment by reverse transcription nested PCR The PCR amplified fragment with restrict enzyme terminus was inserted the vector to construct the recombinant expression plasmid Result The recombinant polypeptide was yielded in E coli by induced with IPTG Western blotting indicated the recombinant polypeptide Could be recognized and combined by the serum against the dengue virus from the patient infected with the virus Conclusion The truncated envelope antigenic gene has been successfully expressed in E colias a antigen for dengue serum detection" @default.
- W2365202871 created "2016-06-24" @default.
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- W2365202871 date "2003-01-01" @default.
- W2365202871 modified "2023-09-23" @default.
- W2365202871 title "Clone and expression of the truncated envelope antigenic gene in E.coli" @default.
- W2365202871 hasPublicationYear "2003" @default.
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