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- W2365220098 abstract "[Objective] To explore the relation between growth hormone gene sequence with physiology and biology characteristic of Juema swine.[Method] The genome DNA was extracted from the blood of Juema swine.The growth hormone gene sequence of Juema swine was amplifyied through PCR and analyzed.[Result] The length of growth hormone gene sequence in Juema swine was 1 671 bp,and the gene sequence contained 5 exons and 4 introns.The base number of exons 1,2,3,4 and 5 were 10,161,117,162 and 201 bp,respectively,and the base number of introns 1,2,3 and 4 was 244,210,192 and 277 bp,respectively.GT-AG regulation can be found all the gene sequence except the first exon and intron.The order of four kinds bases content in Juema swine's growth hormone gene are GCTA.The contents of(G + C)and(A + T)are more than 62% and 37%,respectively.The forth exon of Juema swine showed missense mutation A→G in +1 046,others were all synonymous mutation.The precursor protein coding by Juema pig's growth hormone gene contained 216 amino acids,and maturation protein was a secretory protein consisting of 190 amino acid residue.It contained 6 α-helix helical structures in the secondary structure.It was a globular protein with loose tertiary structure,which contained 6 α-helix anterior regions.[Conclusion] The research provide reference and basis for researching the growth hormone gene sequence of Juema swine." @default.
- W2365220098 created "2016-06-24" @default.
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- W2365220098 date "2010-01-01" @default.
- W2365220098 modified "2023-09-23" @default.
- W2365220098 title "Cloning and Polymorphism Analysis of Growth Hormone Gene in Juema Swine" @default.
- W2365220098 hasPublicationYear "2010" @default.
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