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- W2365233786 abstract "Objective To study changes of gene profiles of gastric tissue in normal and fasting rats by using cDNA chip.Methods SD rats were used to establish fasting rat models and sacrificed to take RNA in gastric tissues.Northern blot,Wester blotting and real-time PCR were applied to detect expressions of genes of gastric tissue in fasting or normal rats.Results After screening of 9 233 genes or expressed sequence tags(ESTs),504 genes or ESTs were identified showing differential expression in fasting rat gastric tissue,with 263 known genes and 87 known ESTs.Among the differential expression genes,262 genes were up-regulated and 242 genes were down-regulated,which including the genes associated with cell structure and movement and cell signal transduction.The reliability of the chip was 80% through the validation of real-time PCR. Conclusion Gene chip is a good method to detect the different expression genes in fasting rats gastric tissue,which provides us more information for gastric physiology,new energy metabolism and appetite adjustment genes,and new function of genes." @default.
- W2365233786 created "2016-06-24" @default.
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- W2365233786 date "2008-01-01" @default.
- W2365233786 modified "2023-09-24" @default.
- W2365233786 title "Differential analysis on gene profiles of gastric tissues in normal and fasting rats" @default.
- W2365233786 hasPublicationYear "2008" @default.
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