Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365252011> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2365252011 abstract "Objective Establishing two methods for rapid and accurate identification of genotype of phenylketonuria model mouse.Methods A 132 bp fragment containing model mouse pah point mutation was amplified with PCR.The PCR products were digested with restriction enzyme Alw 26I and minute amount of SYBR Green I was added.Then the dissociation curve was analyzed on real-time PCR instrument;the digestion product was electrophoresized on 12% polyacrylamide gel meanwhile.The second technique analyzed the amplified 132 bp fragment by DHPLC (60.9 ℃) and the genotype was identified through profile patterns.Results The dissociation curve showed two peaks at 84 ℃ and 81 ℃ for wild type DNA,two peaks at 76.5-77.5 ℃ and 81 ℃ for homozygous genotype,and three peaks at 84 ℃,81 ℃ and 76.5-77.5 ℃ for heterozygous genotype.These three peak types could be distinguished easily.The results were in consistence with the results of PCR-RFLP.The column temperature was optimized for the DHPLC method.Under the optimum condition,the DHPLC profile pattern showed characteristic peak types:homoduplex peak and a heteroduplex peak.Twenty homozygous genotype mice,22 heterozygous genotype mice and 11 wild genotype mice were detected with these two new established methods.The results of these two methods were consistent with each other and with the classic PCR-RFLP.Conclusions Both the dissociation curve method and the DHPLC method can be used to identify the genotypes of phenylketonuria model mice with high accuracy,and the advantages of automation and high-influx." @default.
- W2365252011 created "2016-06-24" @default.
- W2365252011 creator A5052059979 @default.
- W2365252011 date "2009-01-01" @default.
- W2365252011 modified "2023-09-25" @default.
- W2365252011 title "Use of new methods of rapid genotyping the model mice for phenylketonuria" @default.
- W2365252011 hasPublicationYear "2009" @default.
- W2365252011 type Work @default.
- W2365252011 sameAs 2365252011 @default.
- W2365252011 citedByCount "0" @default.
- W2365252011 crossrefType "journal-article" @default.
- W2365252011 hasAuthorship W2365252011A5052059979 @default.
- W2365252011 hasConcept C102931765 @default.
- W2365252011 hasConcept C104317684 @default.
- W2365252011 hasConcept C128319531 @default.
- W2365252011 hasConcept C134494468 @default.
- W2365252011 hasConcept C135763542 @default.
- W2365252011 hasConcept C147789679 @default.
- W2365252011 hasConcept C153911025 @default.
- W2365252011 hasConcept C168007829 @default.
- W2365252011 hasConcept C185592680 @default.
- W2365252011 hasConcept C2780968160 @default.
- W2365252011 hasConcept C31467283 @default.
- W2365252011 hasConcept C49105822 @default.
- W2365252011 hasConcept C54355233 @default.
- W2365252011 hasConcept C552990157 @default.
- W2365252011 hasConcept C84133368 @default.
- W2365252011 hasConcept C86803240 @default.
- W2365252011 hasConceptScore W2365252011C102931765 @default.
- W2365252011 hasConceptScore W2365252011C104317684 @default.
- W2365252011 hasConceptScore W2365252011C128319531 @default.
- W2365252011 hasConceptScore W2365252011C134494468 @default.
- W2365252011 hasConceptScore W2365252011C135763542 @default.
- W2365252011 hasConceptScore W2365252011C147789679 @default.
- W2365252011 hasConceptScore W2365252011C153911025 @default.
- W2365252011 hasConceptScore W2365252011C168007829 @default.
- W2365252011 hasConceptScore W2365252011C185592680 @default.
- W2365252011 hasConceptScore W2365252011C2780968160 @default.
- W2365252011 hasConceptScore W2365252011C31467283 @default.
- W2365252011 hasConceptScore W2365252011C49105822 @default.
- W2365252011 hasConceptScore W2365252011C54355233 @default.
- W2365252011 hasConceptScore W2365252011C552990157 @default.
- W2365252011 hasConceptScore W2365252011C84133368 @default.
- W2365252011 hasConceptScore W2365252011C86803240 @default.
- W2365252011 hasLocation W23652520111 @default.
- W2365252011 hasOpenAccess W2365252011 @default.
- W2365252011 hasPrimaryLocation W23652520111 @default.
- W2365252011 hasRelatedWork W1700051018 @default.
- W2365252011 hasRelatedWork W1999336249 @default.
- W2365252011 hasRelatedWork W2054334204 @default.
- W2365252011 hasRelatedWork W2100085751 @default.
- W2365252011 hasRelatedWork W2121386278 @default.
- W2365252011 hasRelatedWork W2125037730 @default.
- W2365252011 hasRelatedWork W2335938809 @default.
- W2365252011 hasRelatedWork W2366413640 @default.
- W2365252011 hasRelatedWork W2382313512 @default.
- W2365252011 hasRelatedWork W2387698024 @default.
- W2365252011 hasRelatedWork W2393687153 @default.
- W2365252011 hasRelatedWork W2408404971 @default.
- W2365252011 hasRelatedWork W24141549 @default.
- W2365252011 hasRelatedWork W2419571627 @default.
- W2365252011 hasRelatedWork W2462104217 @default.
- W2365252011 hasRelatedWork W2466312853 @default.
- W2365252011 hasRelatedWork W3028986455 @default.
- W2365252011 hasRelatedWork W3172381192 @default.
- W2365252011 hasRelatedWork W2743932688 @default.
- W2365252011 hasRelatedWork W2830661688 @default.
- W2365252011 isParatext "false" @default.
- W2365252011 isRetracted "false" @default.
- W2365252011 magId "2365252011" @default.
- W2365252011 workType "article" @default.