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- W2365297448 abstract "To explore the significance of fusion proteins of EBV latent membrane protein-2A (LMP2A) antigen in the serological diagnosis for nasopharyngeal carcinoma(NPC),3 pairs of oligonucleotide primers were designed according to the genomic sequences of 4 main antigenic epitopes, the fusion gene containing 4 main LMP2A epitopes was synthesized with overlapping extension PCR and then was cloned into vector pGEX-4T2 for fusion protein to express in E.coli. The recombinant protein was purified by GST chromatography column and this protein was used as antigen in ELISA in the serological diagnosis for NPC patients. In these ways,4 fusion proteins(EC2A) containing 4 main antigenic epitopes of EBV LMP2A antigens with the purities more than 90% were obtained. Tested with ELISA,it was demonstrated that 77.9% of NPC patients showed positive results in their sera,but negative results in health individual controls. Comparing with the anti-viral capsid antigen(VCA)-IgA and LMP2A-IgG titers in sera from 68 NPC patients,9 specimens of sera(13.3%)negative for anti-VCA-IgA were positive for LMP2A-IgG. It is evident that the recombinant fusion EC2A protein provides a suitable antigen for the detection of NPC patients as well as for the preparation of useful monoclonal antibodies." @default.
- W2365297448 created "2016-06-24" @default.
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- W2365297448 date "2008-01-01" @default.
- W2365297448 modified "2023-09-28" @default.
- W2365297448 title "The fusion expression of EBV latent membrane protein-2A antigen epitopes and its significance" @default.
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