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- W2365298129 abstract "Objective:To express the fusion protein of LanCL1 in prokaryotic and eukaryotic cells,and prepare the anti-LanCL1 polyclonal antibody.Methods:The CDS of LanCL1 was amplified by PCR from normal mice,and then subcloned into the GST-fused protein expression vector pGEX-6p-1,After transformed with the vector,the BL21 strains were induced by IPTG.To get high purity protein,we treated the lysates with immobilized Glutathione Sepharose,and got rid of ions by dialysis.The purified protein was analyzed by SDS-PAGE,and then immuned the New Zealand white rabbits to prepare antibody.The antibody titer and specification were indentified by western bolt.Results:The GST-fusion protein of LanCL1 had 90% purity,0.44 mg/mL concentration after series procedures and polyclonal antibody from the rabbit could detect LanCL1 both from the brain and from the eukaryotic cell lysates at a high titer.Conclusion:We had successfully obtained eukaryotic and prokaryotic fusion protein of LanCL1,got high sensitivity and specification anti-LanCL1 polyclonal antibody.That lays a solid foundation for the further studies of LanCL1 function." @default.
- W2365298129 created "2016-06-24" @default.
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- W2365298129 date "2008-01-01" @default.
- W2365298129 modified "2023-09-26" @default.
- W2365298129 title "Expression,Purification of LanCL1 Fusion Protein and Preparation,Identification of Its Polyclonal Antibody" @default.
- W2365298129 hasPublicationYear "2008" @default.
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