Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365316469> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2365316469 abstract "Objective:Recent studies demonstrated that hypoxic injury of renal tubular cell played an important role in the pathogenesis of radiocontrast nephropathy.In this study,we investigated the role of hypoxia-inducible factor-1α(HIF-1α)upregulated by Cobalt Chloride(CoCl2) in iopamidol induced tubular cell apoptosis in vitro.Methodology:Cultured human proximal tubular epithelial cells(HK-2) were divided into three groups:negative control,positive control treated with iopamidol(296 mgI/ml,12 h) and pretreated group which treated with CoCl2(5,25,50 and 100 μmol/L) at different time points(0 h,1 h,2 h,4 h,6 h and 12 h) and then iopamidol(296 mgI/ml,12 h).The proportion and morphologic change of apoptotic cell was examined by flow cytometry and Hoechst 33258 staining.The protein level of HIF-1α,caspase-3 and Bcl-2 in HK-2 cells was determined by Western blot analysis.Results:By flow cytometry,50 μmol/L CoCl2 pre-treated for 4,6 and 12 hours reduced the proportion of apoptotic cell significantly(P0.05).The proportion of apoptotic cells were lowered at 6 h and 12 h time points compared with 4 h time point(P0.05).The proportion of apoptotic cells in CoCl2 pretreated for 6 and 12 hours was similar(P0.05).There was no significant difference in the proportion of apoptotic cell between 50 μmol/L and 100 μmol/L pretreated groups at the same time points(6 h and 12 h,P0.05).By Hoechst 33258 staining,50 μmol/L CoCl2 pretreated for 6 and 12 hours attenuated the morphological changes of cells compared with positive control.Western blot analysis revealed that the level of HIF-1α of HK-2 cells pretreated with 50 μmol/L CoCl2 over 1 hour was significantly increased(P0.05).The level of HIF-1α expression reached the peak value at the time point of 6 hours and then presented descending trend.Compared with positive control,the expression of caspase-3 of HK-2 cells exposed to iopamidol and pretreated with 50 μmol/L CoCl2 for 6 and 12 hours was significantly reduced(P0.05) and the level of Bcl-2 was markedly increased(P0.05).Conclusion:These data indicate that up-regulation of HIF-1α inhibit renal tubular epithelial apoptosis induced by non-ionic iodinated contrast,which may involve the reduction of caspase-3 and up-regulation of Bcl-2." @default.
- W2365316469 created "2016-06-24" @default.
- W2365316469 creator A5038327626 @default.
- W2365316469 date "2008-01-01" @default.
- W2365316469 modified "2023-09-23" @default.
- W2365316469 title "Hypoxia-inducible factor-1α and renal tubular epithelial apoptosis induced by non-ionic iodinated contrast" @default.
- W2365316469 hasPublicationYear "2008" @default.
- W2365316469 type Work @default.
- W2365316469 sameAs 2365316469 @default.
- W2365316469 citedByCount "0" @default.
- W2365316469 crossrefType "journal-article" @default.
- W2365316469 hasAuthorship W2365316469A5038327626 @default.
- W2365316469 hasConcept C104317684 @default.
- W2365316469 hasConcept C126838900 @default.
- W2365316469 hasConcept C142724271 @default.
- W2365316469 hasConcept C1491633281 @default.
- W2365316469 hasConcept C153911025 @default.
- W2365316469 hasConcept C16685009 @default.
- W2365316469 hasConcept C178790620 @default.
- W2365316469 hasConcept C185592680 @default.
- W2365316469 hasConcept C190283241 @default.
- W2365316469 hasConcept C202751555 @default.
- W2365316469 hasConcept C2776415932 @default.
- W2365316469 hasConcept C2776982972 @default.
- W2365316469 hasConcept C2779786684 @default.
- W2365316469 hasConcept C540031477 @default.
- W2365316469 hasConcept C553184892 @default.
- W2365316469 hasConcept C55493867 @default.
- W2365316469 hasConcept C71924100 @default.
- W2365316469 hasConcept C74864618 @default.
- W2365316469 hasConcept C7836513 @default.
- W2365316469 hasConcept C86803240 @default.
- W2365316469 hasConceptScore W2365316469C104317684 @default.
- W2365316469 hasConceptScore W2365316469C126838900 @default.
- W2365316469 hasConceptScore W2365316469C142724271 @default.
- W2365316469 hasConceptScore W2365316469C1491633281 @default.
- W2365316469 hasConceptScore W2365316469C153911025 @default.
- W2365316469 hasConceptScore W2365316469C16685009 @default.
- W2365316469 hasConceptScore W2365316469C178790620 @default.
- W2365316469 hasConceptScore W2365316469C185592680 @default.
- W2365316469 hasConceptScore W2365316469C190283241 @default.
- W2365316469 hasConceptScore W2365316469C202751555 @default.
- W2365316469 hasConceptScore W2365316469C2776415932 @default.
- W2365316469 hasConceptScore W2365316469C2776982972 @default.
- W2365316469 hasConceptScore W2365316469C2779786684 @default.
- W2365316469 hasConceptScore W2365316469C540031477 @default.
- W2365316469 hasConceptScore W2365316469C553184892 @default.
- W2365316469 hasConceptScore W2365316469C55493867 @default.
- W2365316469 hasConceptScore W2365316469C71924100 @default.
- W2365316469 hasConceptScore W2365316469C74864618 @default.
- W2365316469 hasConceptScore W2365316469C7836513 @default.
- W2365316469 hasConceptScore W2365316469C86803240 @default.
- W2365316469 hasOpenAccess W2365316469 @default.
- W2365316469 hasRelatedWork W2121253314 @default.
- W2365316469 hasRelatedWork W2350350605 @default.
- W2365316469 hasRelatedWork W2351117820 @default.
- W2365316469 hasRelatedWork W2352487517 @default.
- W2365316469 hasRelatedWork W2353225338 @default.
- W2365316469 hasRelatedWork W2353331129 @default.
- W2365316469 hasRelatedWork W2354417070 @default.
- W2365316469 hasRelatedWork W2355531672 @default.
- W2365316469 hasRelatedWork W2363984220 @default.
- W2365316469 hasRelatedWork W2364011638 @default.
- W2365316469 hasRelatedWork W2367720836 @default.
- W2365316469 hasRelatedWork W2369728916 @default.
- W2365316469 hasRelatedWork W2374057609 @default.
- W2365316469 hasRelatedWork W2392060979 @default.
- W2365316469 hasRelatedWork W2414898134 @default.
- W2365316469 hasRelatedWork W2528787660 @default.
- W2365316469 hasRelatedWork W2586864661 @default.
- W2365316469 hasRelatedWork W2978877934 @default.
- W2365316469 hasRelatedWork W3031065899 @default.
- W2365316469 hasRelatedWork W3031346226 @default.
- W2365316469 isParatext "false" @default.
- W2365316469 isRetracted "false" @default.
- W2365316469 magId "2365316469" @default.
- W2365316469 workType "article" @default.