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- W2365363609 abstract "Objective: To study the effects of PAR-2 agonists SLIGKV and tc-LIGRLO,trypsin and its inhibitors on intracellular calcium concentration [Ca~(2+)]_i in the H292 lung epithelial cell.Methods: Fluo-3/AM fluorescence technique and laser scanning confocal microscope(LSCM) were used to measure the intracellular calcium level after different reagents were added in Hanks of H292 cells.Results: Trypsin,SLIGKV,tc-LIGRLO could increase the intracellular calcium concentration [Ca~(2+)]_i in H292 cells.Compared with control,the average fluorescent intensities were increased 267%,60%,37%,respectively.Trypsin inhibitors soybean trypsin inhibitor(SBTI) and α_1-antitrypsin(α_1-AT) could inhibit trypsin induced increase of [Ca~(2+)]_i in H292 cells.Conclusion: Activation of protease-activated receptor-2 induce increase of [Ca~(2+)]_i in H292 cells.Trypsin inhibitors could inhibit trypsin induced the increase of [Ca~(2+)]_i in H292 cells." @default.
- W2365363609 created "2016-06-24" @default.
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- W2365363609 date "2005-01-01" @default.
- W2365363609 modified "2023-09-26" @default.
- W2365363609 title "Effects of Free Calcium in H292 Cells through Protease-activated Receptor-2" @default.
- W2365363609 hasPublicationYear "2005" @default.
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