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- W2365391246 abstract "Streptomyces mobaraensis was used as the original strain. The strain was mutated with ultraviolet,and the mutation time was determined as 50s.A mutant strain MY78with the higher capacity for enzymatic production was obtained.Compared with the original strain(transglutaminase activity was 0.25U/mL),the transglutaminase activity produced by the mutant strain increased 2.28times. The mutant strain had genetic stability.The mutant strain was used to ferment to prepare transglutaminase by shake flask. The optimizedfermentation conditions were as followed:initial pH 7.0, inoculum size 10%(V/V),temperature 30℃,shaking speed 200r/min,and fermentation time 54h.In the conditions,the transglutaminase activity and biomass of the strain were(1.15± 0.09)U/mL and(6.32±0.01)g/L,respectively." @default.
- W2365391246 created "2016-06-24" @default.
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- W2365391246 date "2013-01-01" @default.
- W2365391246 modified "2023-09-25" @default.
- W2365391246 title "Breeding of producing transglutaminase strains and optimizing fermentation conditions" @default.
- W2365391246 hasPublicationYear "2013" @default.
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