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- W2365397312 abstract "Objective To isolate Lactobacillus strain with high adherence from human gastrointestinal tract for clinical use. Methods Potentially probiotic Lactobacillus strains were isolated using a modified LAMVAB selective medium from human intestinal mucosa samples collected by gastrointestinal biopsy, then subjected to physiological and biochemical identification as well as 16S rRNA sequencing, and tested for acid- and oxgall-resistances, adhesive function and antimicrobial activity. Results An Lactobacillus strain L2 was isolated, which could grow in modified LAMVAB selective medium and showed resistance to acid (pH 2. 5) and 0. 3% oxgall. In vitro adhesion test showed that the adhesion indexes of L2 strain to human enteric epithelium-like cells Caco-2 and rat intestinal epithelial cells IEC-6 were (595 ± 125. 76)/100 cells and (538 ± 65. 2)/100 cells respectively, and the adhesion was calcium ion-dependent. L2 strain showed strong inhibitory effect on pathogen indicators such as E. coli and Pseduomonas aeruginosa, but no inhibitory effect on probiotics such as Lactobacillus rhamnosus LGG and Lactobacillus acidophilus L050103-12. The strain was identified as Lactobacillus plantarum. Conclusion The isolated Lactobacillus plantarum L2 showed good adhesion, acid- and oxgall-resistances and antimicrobial activity, and might be used as a novel probiotics." @default.
- W2365397312 created "2016-06-24" @default.
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- W2365397312 date "2008-01-01" @default.
- W2365397312 modified "2023-09-28" @default.
- W2365397312 title "Isolation and Identification of An Adhesive Probiotic Lactobacillus Strain from Human Gastrointestinal Tract" @default.
- W2365397312 hasPublicationYear "2008" @default.
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