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- W2365402868 abstract "Objective To establish high performance liquid chromatography(HPLC)method for the determination of the content of gliclazide in dog plasma.Methods The chromatographic conditions were as follow:Nucleodur C18 kieselgel column(250×4.6 mm2,Φ5μm)as chromatographic column;phosphate buffer(pH2.5)methanol(40:60,v/v)as mobile phase;the flow rate:1.0 mL/min;the detection wavelength:228 nm;tolbutamide selected as an internal standard.Results The calibration curve was linear(r=0.999 9)in the concentration range of 0.125-16μg/mL for gliclazide.The limit of detection(LOD)was 0.10 μg/mL.The RSD of inter-and intra-day were less than 8%.The average extraction recovery rate was 79.98%.Conclusion HPLC assay is simple,rapid and sensitive,suitable for the pharmacokinetic study of gliclazide." @default.
- W2365402868 created "2016-06-24" @default.
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- W2365402868 date "2008-01-01" @default.
- W2365402868 modified "2023-09-26" @default.
- W2365402868 title "Determination of the content of gliclazide in dog plasma by high performance liquid chromatography assay" @default.
- W2365402868 hasPublicationYear "2008" @default.
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