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- W2365403002 abstract "[Objective] To construct the prokaryotic expression vector of the movement protein gene of barley yellow dwarf virus(BYDV-MP) and efficiently express the movement protein in E.coli-BL21.[Method] According to movement protein gene sequence of the Barley Yellow Dwarf Virus(BYDV) PAV6 in Genebank,a pair of primers were designed and synthesized,BYEDV-MP gene was amplified from infected wheat leaves by RT-PCR,pGEX-4T-1-MP was constructed by using recombinant DNA technology and then expressed in E.coli-BL21(DE3).[Result] The construct of pGEX-4T-1-MP was verified by restriction endonuclease and sequenced.Compared with the sequence of movement protein genes of The Barley Yellow Dwarf Virus(BYDV) in the GeneBank,their homology was 100%.The expression of the GST-MP fusion protein was induced with IPTG.Its molecular weight was about 43 KDa,the results was identified by electrophoreses of SDS-PAGE,the expression of protein bands was consistent with the expected size.[Conclusion] The recombinant prokaryotic expression vector in pGEX-4T-1-MP was constructed successfully and highly expressed in E.coli-BL21,which laid a foundation for the further study on function and structure of MP." @default.
- W2365403002 created "2016-06-24" @default.
- W2365403002 creator A5085107837 @default.
- W2365403002 date "2011-01-01" @default.
- W2365403002 modified "2023-09-24" @default.
- W2365403002 title "Cloning and Expression of Movement Protein Gene of Barley Yellow Dwarf Virus(BYDV-MP)" @default.
- W2365403002 hasPublicationYear "2011" @default.
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