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- W2365411617 abstract "【Objective】To observe the changes of cell-mediated immunity and the effect of glutamine (Gln) on them in severe acute pancreatitis (SAP).【Methods】Seventy-two patients with SAP were randomized equally into 2 groups and received standard total parenteral nutrition (TPN) with intravenous infusion of Gln or normal saline (control) for 1 week. The blood samples were collected one day before and on day 7 of Gln administration. The human leukocyte antigen-DR (HLA-DR) bound on CD14+ mononuclear cell surface of SAP patients was quantified by flow cytometry. The ability of T lymphocyte proliferation was determined by thiazolyl blue (MTT) method, and inter-leukin-2(IL-2) release was measured by enzyme-linked immunosorbent assay (ELISA). 16 healthy person were chosen as the healthy group. 【Results】Compared to the healthy group, the expression of HLA-DR on CD14+ mononuclear cell surface, the T lymphocyte proliferative activity and IL-2 release in all enrolled SAP patients before treatment were significantly decreased. After Gln treatment, of all the above-mentioned index tended to normal, but there was progressively decreased in non-treatment. 【Conclusions】The cellular immune function is suppressed in SAP patients, the manifest are the suppression of T lymphocyte proliferation, IL-2 release and the expression of HLA-DR on CD14+ mononuclear cell surface. Prophase Gln treatment can help to improve the cellular immune function." @default.
- W2365411617 created "2016-06-24" @default.
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- W2365411617 date "2009-01-01" @default.
- W2365411617 modified "2023-09-23" @default.
- W2365411617 title "Effect of glutamine on the cellular immunity of severe acute pancreatitis" @default.
- W2365411617 hasPublicationYear "2009" @default.
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