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- W2365431294 abstract "Objective To establish and identify a new rat hepatic stellate cell line (rHSC|99). Methods Liver in vivo perfusion of collagenase and density gradient centrifugation were used to isolate hepatic stellate cell from a normal rat.Cells were maintained in culture for multiple passages.Cell proliferation and morphological characterization were studied on 60 passage of HSC.Immunocytochemistry and RT|PCR were used to detect the expression of demin,GFAP,α|SMA,collagen type Ⅰ and Ⅲ,iNOS,ICAM|1,TGF|α,IL|10 and TGF|α in 60 passage of HSC.Sixty passage cells were cultured on silicone membrane to investigate the cell contraction.Gimsa stain method was used to analyze karyotype. Results rHSC|99 cell has undergone 78 passages.It's morphological characteristics resemble myofibroblast cell.The cell doubing time is 36^5 hours.Immunocytochemistry showed that Demin,GFAP,α|SMA,collagen type Ⅰ and Ⅲ,iNOS,ICAM|1,TGF|α were expressed in rHSC|99 cells.RT|PCR analysis showed that TGF|β and ICAM|1 were expressed in rHSC|99 cells.Cell contraction was induced by ET|1.Cell clone was not formed after 14 days culture in soft agar.rHSC|99 cells displayed normal rat karyotype with 42 chromosomes in modal number.Conclusion rHSC|99 cell line exhibits characteristics of activated hepatic stellate cell.It will be a useful tool to study the pathogenesis of liver cirrhosis and portal hypertension.;[" @default.
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- W2365431294 date "2003-01-01" @default.
- W2365431294 modified "2023-09-23" @default.
- W2365431294 title "ESTABLISHMENT OF A NEW RAT HEPATIC STELLATE CELL LINE rHSC-99 WITH CHARACTERISTICS OF MYOFIBROBLAST CELL" @default.
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