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- W2365434427 abstract "Objective To establish a simple,sensitive and rapid HPLC method for determination of clozapine in human plasma.Methods Clozapine and was extracted from plasma with ether,chromatographic analysis was carried on a Luna C18(250×4.6mm,5μm) column and the column temperature was 40℃.The mobile phase consists of Acetonitrile-Ammonium acetate(46:54,V/V),the flow rate was 0.9 mL/min,UV detector was set at 243nm,and Estazolam was used as the internal standard.Results A linearity was obtained in the range of 0.05~1.60μg/mL(r=0.9992,n=6),the lowest detectable concentration in plasma was 0.01μg/mL(S/N=3),the recovery of low,medium and high concentration were within 98.8%~104.0%,The relative standard deviations of intra-day and inter-day assay were within 2.96%~11.50%.Conclusion The method is rapid,simple and accurate,which can be use for determination of clozapine levels in human plasma." @default.
- W2365434427 created "2016-06-24" @default.
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- W2365434427 date "2008-01-01" @default.
- W2365434427 modified "2023-09-23" @default.
- W2365434427 title "Determination of Clozapine in Human Plasma by RP-HPLC" @default.
- W2365434427 hasPublicationYear "2008" @default.
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