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- W2365435768 abstract "Objective To study the pathogenesis of HCV and the biological functions of a novel HCV non-structural protein 3 transregulated protein(NS3TP2),we investigated the gene expression of NS3TP2 in yeast.Methods PCR was performed to amplify the gene of NS3TP2,and the gene was cloned into pGEM-T vector.The gene of NS3TP2 was cut from pGEM-T vector and cloned into yeast expression plasmid pGBKT7,then pGBKT7-NS3TP2 was transformed into yeast AH109.The yeast protein was isolated and analyzed by sodium dodecy1 sulfate-polyaxry lamide Gel electrophoresis(SFS-PAGE)and western blotting assay.Results NS3TP2 gene was successfully cloned into pGBKT7.NS3TP2 exists within yeast cells.Conclusison The findings suggest that NS3TP2 is successfully expressed in yeast system." @default.
- W2365435768 created "2016-06-24" @default.
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- W2365435768 date "2007-01-01" @default.
- W2365435768 modified "2023-09-25" @default.
- W2365435768 title "Cloning and expression of gene of NS3TP2 in yeast" @default.
- W2365435768 hasPublicationYear "2007" @default.
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