Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365441969> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2365441969 abstract "Mucosally induced tolerance is an attractive strategy for immunotherapy of autoimmune diseases.In this study,A recombinant expression plasmid pET28a-CTB-GADⅢ was construcated in which glutamic acid decarboxylase 65 derived peptides(p217-236,p524-538,p290-306) genes were fused to the 3' end of cholera toxin B subunit gene.Subsequently the recombinant plasmid was transformed into E.coli strain BL21(DE3).The recombinant protein CTB-GADⅢ was expressed and accumulated as inclusion bodies after being induced with 5 mmol/L lactose for 5 h.Purificated recombinant protein was obtained after washing,renaturing and purifying via CM52-cellulose and sephadex G100 chromatography.The protein was further analyzed for GM1 binding activity in a GM1-ELISA.It showed good GM1-binding,although not as strong as cholera toxin B subunit.In addition,the CTB-GADⅢ fusion protein induces efficient immunosuppression after nasal administration of nonobese diabetic mice,raising the possibility of using such construct in the treatment of type 1 diabetes." @default.
- W2365441969 created "2016-06-24" @default.
- W2365441969 creator A5001792962 @default.
- W2365441969 date "2009-01-01" @default.
- W2365441969 modified "2023-09-25" @default.
- W2365441969 title "Genetic Fusion of Glutamic Acid Decarboxylase 65 Derived Peptides to the B-Subunit of Cholera Toxin and Its Retardation Effect of Diabetes in NOD Mice" @default.
- W2365441969 hasPublicationYear "2009" @default.
- W2365441969 type Work @default.
- W2365441969 sameAs 2365441969 @default.
- W2365441969 citedByCount "0" @default.
- W2365441969 crossrefType "journal-article" @default.
- W2365441969 hasAuthorship W2365441969A5001792962 @default.
- W2365441969 hasConcept C104292427 @default.
- W2365441969 hasConcept C104317684 @default.
- W2365441969 hasConcept C111829193 @default.
- W2365441969 hasConcept C123894998 @default.
- W2365441969 hasConcept C153911025 @default.
- W2365441969 hasConcept C159654299 @default.
- W2365441969 hasConcept C181199279 @default.
- W2365441969 hasConcept C185592680 @default.
- W2365441969 hasConcept C203014093 @default.
- W2365441969 hasConcept C2777006276 @default.
- W2365441969 hasConcept C2777367657 @default.
- W2365441969 hasConcept C2780868878 @default.
- W2365441969 hasConcept C40767141 @default.
- W2365441969 hasConcept C55493867 @default.
- W2365441969 hasConcept C86803240 @default.
- W2365441969 hasConcept C89423630 @default.
- W2365441969 hasConcept C9497952 @default.
- W2365441969 hasConceptScore W2365441969C104292427 @default.
- W2365441969 hasConceptScore W2365441969C104317684 @default.
- W2365441969 hasConceptScore W2365441969C111829193 @default.
- W2365441969 hasConceptScore W2365441969C123894998 @default.
- W2365441969 hasConceptScore W2365441969C153911025 @default.
- W2365441969 hasConceptScore W2365441969C159654299 @default.
- W2365441969 hasConceptScore W2365441969C181199279 @default.
- W2365441969 hasConceptScore W2365441969C185592680 @default.
- W2365441969 hasConceptScore W2365441969C203014093 @default.
- W2365441969 hasConceptScore W2365441969C2777006276 @default.
- W2365441969 hasConceptScore W2365441969C2777367657 @default.
- W2365441969 hasConceptScore W2365441969C2780868878 @default.
- W2365441969 hasConceptScore W2365441969C40767141 @default.
- W2365441969 hasConceptScore W2365441969C55493867 @default.
- W2365441969 hasConceptScore W2365441969C86803240 @default.
- W2365441969 hasConceptScore W2365441969C89423630 @default.
- W2365441969 hasConceptScore W2365441969C9497952 @default.
- W2365441969 hasLocation W23654419691 @default.
- W2365441969 hasOpenAccess W2365441969 @default.
- W2365441969 hasPrimaryLocation W23654419691 @default.
- W2365441969 hasRelatedWork W1740335278 @default.
- W2365441969 hasRelatedWork W1902865169 @default.
- W2365441969 hasRelatedWork W1979456422 @default.
- W2365441969 hasRelatedWork W1993816013 @default.
- W2365441969 hasRelatedWork W2005296768 @default.
- W2365441969 hasRelatedWork W2007164958 @default.
- W2365441969 hasRelatedWork W2052550491 @default.
- W2365441969 hasRelatedWork W2082392421 @default.
- W2365441969 hasRelatedWork W2106226571 @default.
- W2365441969 hasRelatedWork W2108819576 @default.
- W2365441969 hasRelatedWork W2115009099 @default.
- W2365441969 hasRelatedWork W2117096569 @default.
- W2365441969 hasRelatedWork W2136513103 @default.
- W2365441969 hasRelatedWork W2152244146 @default.
- W2365441969 hasRelatedWork W2159364591 @default.
- W2365441969 hasRelatedWork W2316792229 @default.
- W2365441969 hasRelatedWork W2409832637 @default.
- W2365441969 hasRelatedWork W2418264438 @default.
- W2365441969 hasRelatedWork W3134050005 @default.
- W2365441969 hasRelatedWork W58970155 @default.
- W2365441969 isParatext "false" @default.
- W2365441969 isRetracted "false" @default.
- W2365441969 magId "2365441969" @default.
- W2365441969 workType "article" @default.