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- W2365452710 abstract "Objective In order to seek for a therapeutic approach to the diseases caused by HPV or cervix cancer,we constructed antisense eukaryotic fluorescent expression vector of human papillomavirus(HPV)18 E6E7 and detected their expression in HeLa cervical carcinoma cells.Methods HPV18 E6E7 716bp was amplified the by PCR with the full-length HPV-18 cDNA clone vector as the template.Then the PCR product was inserted into eukaryotic fluorescent expressing vector pEGFP in antisense orientation by DNA recombinant technology and further transfected into HeLa cervical carcinoma cells.Expression of HPV18 E6/E7 in transfectants was examined by the fluorescence microscope,RT-PCR and Western blot.Results The fluorescence eukaryotic expression vector carrying antisense HPV18 E6E7 gene fragment was successfully constructed.At 48h after transfection of recombinant plasmid,bright green fluorescence was visible and the E6/E7 mRNA and proteins were obviously down regulated in the HeLa cervical carcinoma cells.Conclusion Delivery of antisense HPV18 E6E7 can effectively suppress the expression of E6/E7 oncogenes and is potentially a new approach to treat HPV infection and cervix cancer." @default.
- W2365452710 created "2016-06-24" @default.
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- W2365452710 date "2007-01-01" @default.
- W2365452710 modified "2023-09-23" @default.
- W2365452710 title "Construction of Antisense Human Papillomavirus (HPV) 18 E6E7 and Green Fluorescent Protein (EGFP) Eukaryotic Co-expression Vector and Its Expression in HeLa Cervical Carcinoma Cells" @default.
- W2365452710 hasPublicationYear "2007" @default.
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