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- W2365455998 abstract "AIM: To construct a eukaryotic expressing vector of sense and antisense human Tankyrase gene. METHODS : The human Tankyrase cDNA fragment contained in the TT20 vector was cloned into the eukaryotic expressing vector pcDNA3.1 in cis direction or trans direction using DNA recombinant technology. The recombinant vector was further identified by digestion of Bam H Ⅰ. RESULTS: After digested by Bam HⅠ, two fragments which lengthened 5.0 and 3.4 kb were formed in sense eukaryotic expressing vector (pcDNA TNKS), while two fragments which lengthened 8.2 kb and 0.2 kb were formed in antisense vector (pcDNA aTNKS). Electrophoretic results were completely coincident with theoritical calculation. CONCLUSION: Human Tankyrase sense and antisense eukaryotic expressing vectors are successfully constructed." @default.
- W2365455998 created "2016-06-24" @default.
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- W2365455998 date "2003-01-01" @default.
- W2365455998 modified "2023-09-23" @default.
- W2365455998 title "Construction and identification of sense and antisense human Tankyrase eukaryotic expression vector" @default.
- W2365455998 hasPublicationYear "2003" @default.
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