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- W2365488168 abstract "Objective To understand the sensitivity of the gene expression of heat shock protein70(HSP70) induced by cadmium and whether it can be taken as a biomarker in cadmium exposure assessment.Methods L02 cells were exposed to 0.5,1.0 and 2.0 μmol/L of CdSO4 for 4 h,then the expression of HSP70 mRNA was observed by polymerase chain reaction(PCR).Wistar rats aged 6 weeks were divided into 4 groups,16 in each,including 8 male and 8 female.The rats in the experimental groups were treated with CdSO4 by gavage once a day at the doses of 25.0 mg/kg,8.0 mg/kg and 3.0 mg/kg respectively for 28 consecutive days,one group was the negative control.After the exposure,the liver tissue was sampled to examine the expression of HSP70 mRNA in the liver tissue by PCR.Results In the CdSO4-treated groups(25.0 mg/kg,8.0 mg/kg),the expression of HSP70 mRNA in the liver tissue was significantly up-regulated(P0.05) with a dose-dependent manner compared with the negative control group.Conclusion CdSO4 may induce an up-regulated expression of HSP70mRNA in the hepatic tissue and in L02 cells in rats." @default.
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- W2365488168 date "2011-01-01" @default.
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- W2365488168 title "Gene expression of heat shock protein 70 induced by cadmium in rats." @default.
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