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- W2365513621 abstract "Objective:Study the effect of DNA purification after extraction from formalin fixed and paraffin embedded tissues(FFPET)on PCR amplification efficiency.Methods:Compared the PCR amplification efficiency of DNA samples extracted from formalin fixed and paraffin embedded tissues and the DNA samples further purified by UNIQ-10 spin column after extraction using specific primers and optimized PCR conditions for β-globulin(110 bp).Results:Further purification of DNA samples with UNIQ-10 spin column after DNA extraction increased PCR amplification efficiencies for the gene studied in all samples were compared to the DNA samples without further purification step.Conclusion:Further purification of DNA samples with UNIQ-10 spin column after extraction removes the PCR inhibiting factors that could not be removed by proteinase K in the DNA templates from formalin fixed and paraffin embedded tissues,and increases the sensitivity of PCR amplification." @default.
- W2365513621 created "2016-06-24" @default.
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- W2365513621 date "2008-01-01" @default.
- W2365513621 modified "2023-09-22" @default.
- W2365513621 title "Further study on the quality of DNA extracted from formalin fixed and paraffin embedded tissues" @default.
- W2365513621 hasPublicationYear "2008" @default.
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