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- W2365525692 abstract "Objective To evaluate the sensitivity and specificity of the molecular biology method which combining the universal primer PCR and arbitrary primer PCR for the detection of pathogenic dermatophytes in laboratory animals,and validate the feasibility of the method through building animal models of pathogenic dermatophytes in laboratory animals and practical detection of dermatophytes.Methods The determination of the specificity: E.coli DNA,Canine hepatitis virus DNA and mouse hepatoma carcinoma H_(22) cell DNA was amplified by universal primers.The determination of the sensitivity: DNA weight concentration was determined by UV-vis spectroscopy and it was further diluted by series of ten times weight concentration based upon the former measurement.The resulting eight weight concentrations ranging from 100 ng～10 fg were used for further PCR amplification.Then build the animal models and identify some skin samples of laboratory animals from Hebei province.Results Amplification of the three standard strains DNA with universal primers yielded the same band(about 440 bp).While E.coli,Canine hepatitis virus,mouse hepatoma carcinoma H_(22) cell were not amplificated with strains.Sensitivity determination of universal primer PCR: Seven samples ranging from 100 ng to 100 fg shown positive,except the 10 fg sample yielding negative band.We founded the animal models of pathogenic dermatophytes in laboratory animals.For one of the skin samples of laboratory animals from Hebei province,the universal primer PCR amplification yielded a 440 bp band.Conclusions The universal primer(CHS1 1S,CHS1 1R)is regarded as a specificity primer.The result showed that the sensitivity of universal primer PCR is 100 fg.Combining universal primer PCR and AP-PCR could differentiate the three familiar dermatophytes in laboratory animals." @default.
- W2365525692 created "2016-06-24" @default.
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- W2365525692 date "2007-01-01" @default.
- W2365525692 modified "2023-09-25" @default.
- W2365525692 title "Testing Lab Animal Dermatophytes Using PCR Method" @default.
- W2365525692 hasPublicationYear "2007" @default.
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