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- W2365533049 abstract "Objective To obtain stable labelling of mesenchymal stem cells(MSCs) mediated by recombinant replication-deficient retroviral vector expressing green fluorescent protein(Rt-GFP) and provide a basis for in vivo differentiation study of MSCs in future.Methods Rat MSCs were cultured and purified with whole bone marrow method.The purified MSCs were infected with Rt-GFP.The GFP positive MSCs were observed with fluorescent microscopy.Results After Rt-GFP infection,80%~90% MSCs were labelled by GFP.The fluorescent intensity of labelled MSCs was not changeable with the prolonged culture time and the increase of passage.Conclusion MSCs may be stably labelled by Rt-GFP." @default.
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- W2365533049 date "2005-01-01" @default.
- W2365533049 modified "2023-09-25" @default.
- W2365533049 title "The labelling of mesenchymal stem cells mediated by recombinant replication-deficient retrovirus expressing green fluorescent protein" @default.
- W2365533049 hasPublicationYear "2005" @default.
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