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- W2365591942 abstract "Objective To establish a simple,economical and efficient method for osteoblasts culture.Methods Osteoblasts were isolated from the skull of neonatal mice by improved tissue explant approach;identified and compared by cell morphology,proliferation,alkaline phosphatase staining,mineralized nodules with alizarin red staining and the expression of osteocalcin(OC) mRNA as well.Results The growth of osteoblasts is in good condition and high purity,with typical osteoblasts morphological features,alkaline phosphatase staining and mineralized nodules alizarin red staining were positive,the expression of OC mRNA obviously was higher than the negative control which presented a statistical difference of significance(P0.05).No significant difference was found in morphology,proliferation or differentiating function of the osteoblasts which were harvested from the 1st~4th series explant cultures(P0.05).Conclusion High purity of mouse osteoblasts derived from the skull of neonatal mice by the improved tissue explant,which reduces money and time and is easy to operate." @default.
- W2365591942 created "2016-06-24" @default.
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- W2365591942 date "2013-01-01" @default.
- W2365591942 modified "2023-09-26" @default.
- W2365591942 title "Studies of Improved Tissue Culturing Mouse Osteoblasts" @default.
- W2365591942 hasPublicationYear "2013" @default.
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