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- W2365620963 abstract "Objective:To study the expression of recombinant anti HBx single chain phage antibody.Methods:The anti HBx single chain phage antibody(pScFv) was constructed for the study on active expression of genetic antibody in E.coli.The anit HBx ScFv gene was recombined with phagemid vector (pCANTAB) and transferred into host bacteria.Results:The anti HBx pScFv was induced with helper phage M13KO7.The soluble expression products fused with phage minor coat protein was secreted which was detected specific binding affinity by Western blot and ELISA assay.Conclusion:The successful construction and expression of anti HBx pScFv might establish the method for the active selecting and preparing the specific antibodies in the E.coli expression system." @default.
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- W2365620963 date "1999-01-01" @default.
- W2365620963 modified "2023-09-25" @default.
- W2365620963 title "Expression of recombinant anti HBx single chain phageantibody" @default.
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