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- W2365627252 abstract "Objective To cultivate,identify and sort bronchoalveolar stem cells(BASC)derived from normal adult mouse lung.Methods After enzymatic digestion of lung tissue with dispase and collagenase in combination,the Sca-1+ cells were isolated from the obtained pulmonary cells by magnetic cell sorting.These Sca-1+ cells were cultured in dishes coated with collagen and mouse fibroblast cell line Swiss-3T3 under a serum-free culture system for BASC,which were identified by the dual-color immunofluorescent staining clara cell specific antigen(CCA)and surfactant protein C(SP-C).Finally,these pure BASC were isolated by the flow cytometry.Results One lung of normal adult mouse could yield(1.6-1.8)×107 nucleated cells in this enzyme digestion procedure.The percentage of Sca-1+ cells we sorted from lung tissue was much higher than the unsorted [(87.3±5.9)% and(9.6±1.8)%,P0.05].On the 4th day of culture,the Sca-1+ cells grew into BASC and other cell colonies.The CD45-CD31-Sca-1+ CD34+cells accounting for(0.7-1.1)% of the total number of pulmonary cells were isolated by flow cytometry.Conclusion Sca-1+ cells derived from adult mice pulmonary cells can grow into BASC in serum-free culture system,and BASC can be purified by flow cytometry.The established platform provides implications for the research of the lung tumor stem cells from human." @default.
- W2365627252 created "2016-06-24" @default.
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- W2365627252 date "2008-01-01" @default.
- W2365627252 modified "2023-09-25" @default.
- W2365627252 title "Cultivation,identification and sorting of bronchoalveolar stem cells derived from mouse lung" @default.
- W2365627252 hasPublicationYear "2008" @default.
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