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- W2365670537 abstract "A high efficient regeneration system of hypocotyls explants in Daucus carota L.var.sativa based on the study of hormones composition and variety of addition materials in medium was developed. This study offers a good regenesis and transformation technical plat to produce carrot oral vaccine. First carrot callus was induced in high 2, 4D concentration media, then the somatic embryoids were induced in lower 2,4D concentration. If adding 0.2wt% yeast extract in this media, the embryoid inductivity can be raised up to 78.27%. Based on the regenesis system we transformed the carrot hypocotyls through Agrobacterium mediated transformation. The results indicated that the regenerated resistant plants could be obtained by pre-culturing for 96 h, co-culturing for 72 h, then transferring to the callus inducement media containing 20 mg·L -1 Kam for 20 d and at last transferring to embryoid inducement media containing 75 mg·L -1 Kam. We detected the resistant plant by means of PCR, and found that 80% Kam resistant plants could be detected the reporter gene-nptII, indicated that the foreign gene was transformed in the plants." @default.
- W2365670537 created "2016-06-24" @default.
- W2365670537 creator A5087356446 @default.
- W2365670537 date "2005-01-01" @default.
- W2365670537 modified "2023-09-25" @default.
- W2365670537 title "High Efficient Regeneration and Agrobacterium Mediated Transformation of Daucus carota L.var.sativa" @default.
- W2365670537 hasPublicationYear "2005" @default.
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